an Papillomavirus Seropositivity Synergizes with MDM2 iants to Increase the Risk of Oral Squamous

nloaded increasing incidence of oral squamous cell carcinoma (OSCC) in young adults has been associated with ly transmitted infections of human papillomavirus (HPV), particularly HPV16. Given the roles of p53 in suppression and of HPV E6 and MDM2 oncoproteins in p53 degradation, we evaluated HPV16 L1 sitivity and MDM2 promoter variants to examine their possible associations with OSCC risk in a ontrol study of 325 patients and 335 cancer-free matched controls. Compared with individuals having -rs2279744 GT or GG genotypes and HPV16 L1 seronegativity, the TT genotype and HPV16 L1 seronegwere found to be associated with an odds ratio (OR) of 1.25 [95% confidence interval (CI), 1.06–2.19] for risk, and GT/GG and HPV16 L1 seropositivity were associated with an OR of 2.81 (95% CI, 1.67–4.74). ose with both the TT genotype and HPV16 L1 seropositivity, the associated OR was 5.57 (95% CI, 2.93– Similar results were observed for the MDM2-rs937283 polymorphism. Moreover, there was a borderline cant or significant interaction between the individual or combined MDM2 genotypes of the two polyisms and HPV16 L1 seropositivity (Pint = 0.060 for MDM2-rs2279744, Pint = 0.009 for MDM2-rs937283, int = 0.005 for the combined MDM2 genotypes) on risk of OSCC. Notably, that effect modification was ularly pronounced in never smokers and never drinkers, and for oropharyngeal as opposed to oral cavity cancer. Taken together, our results indicate that the risk of OSCC associated with HPV16 L1 seropositivity is modified by MDM2 promoter polymorphisms. Cancer Res; 70(18); OF1–10. ©2010 AACR.